Journal: Cells
Article Title: Partial Inhibition of Epithelial-to-Mesenchymal Transition (EMT) Phenotypes by Placenta-Derived DBMSCs in Human Breast Cancer Cell Lines, In Vitro
doi: 10.3390/cells13242131
Figure Lengend Snippet: Effect of cancer cell treatment on the expression of chemokines and cytokines on DBMSCs: Flow cytometry analysis for expression of chemokines and cytokines by DBMSCs after co-culture with DBMSCs showed a significant increase in IL-21, IFNA2, TNSF13B, and IL-17R for 96 h ( A ) but a significant decrease in the expression level of IL-27. The mean fluorescence index (MFI) is plotted and shown as a bar diagram ( B ). Bars represent standard errors. * p < 0.05.
Article Snippet: Fluorescent-labelled antibodies for flow cytometry experiments, including Human Snail Alexa Fluor ® 488-conjugated Antibody, Cat# IC3639G; Human/Mouse/Rat Vimentin APC-conjugated Antibody, Cat# IC2105A; Human Fibronectin PE-conjugated Antibody, Cat# IC1918P; Human/Mouse Wnt-5a Antibody (Cat# MAB645); Human IL-27 PE-conjugated Antibody, Cat# IC25261P; Human BAFF/BLyS/TNFSF13B PE-conjugated Antibody, Cat# IC1241P, Human IL-17RA/IL-17R Antibody; Cat# MAB177; Mouse F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody, Cat# F0103B; Rabbit IgG Fluorescein-conjugated Antibody, Cat# F0112; and Goat IgG (H+L) Fluorescein-conjugated Antibody, Cat# F0109, were purchased from R&D Systems (Minneapolis, MN, USA).
Techniques: Expressing, Flow Cytometry, Co-Culture Assay, Fluorescence